Evolutionary trees from DNA sequences: a maximum likelihood approach. Notification that new names and new combinations have appeared in volume 62, part 3, of the IJSEM. The use of gyrB sequence analysis in the phylogeny of the genus Amycolatopsis. A multigene approach to phylogenetic analysis using the genus Mycobacterium as a model.

Devulder, G., Pe´rouse de Montclos, M., Flandrois, J.P.

Clinical and Laboratory features of the Nocardia spp.

Brown-Elliot, B.A., Brown, J.M., Conville, P.S., Wallace, R.J.

speluncae the similarity of 95.5 and 95.3%., respectively takedensis and had 96.7% similarity, FGN17 and FGN19 isolates were determined to be related with N.

It was determined that FSN27 isolate was closely related with N. rhamnosiphila 97.9, 95.3 and 98.6% sequences similarity, respectively. FSN35, FMN06 and FMN15 Nocardia isolates were determined to be related with N. FSN13, FSN14, FSN34 and FSN37 isolates shared 95.4- 96.4% of the concatenated gyrB-16S rRNA-secA1-rpoB sequences similarity with the type strain N. Concatenate sequence analysis of 2.061 bp allowed differentiation of all isolates and type strains, with a range of interspecies similarity of 91.4-99.8%. The region of gyrB, 16S rRNA, secA1 and rpoB genes of ten isolates were compared with related type strains of Nocardia. Four multilocus gene regions, gyrase B, β subunit of DNA topoisomerase gyrB, 16S rRNA 16S rDNA, subunit A of SecA preprotein translocase secA1, and RNA polymerase rpoB, were used to identify ten Nocardia strains isolated from different soil samples. In this study, identification of the possible novel Nocardia isolates was made by Multilocus Sequence Analysis. Identification of Nocardia strains is difficult due to the failure of 16S rRNA sequence analysis, chemotaxonomical and biochemical tests.

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